Connective tissue metabolism in chikungunya patients

biomed - Lokireddy Sudarsanareddy , Vemula Sarojamma , Vadde , Vadde Ramakrishna

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5 pages

English

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Chikungunya (CHIK) fever is a viral disease transmitted to humans by the bite of Chikungunya virus (CHIK virus) infected Aedes mosquitoes. CHIK virus is a member of the Alphavirus genus of the family Togaviridae . Previous reports have indicated that infection with CHIK virus produces an acute arthritis in human hosts by large area of necrosis and collagenosis or fibrosis. Results We carried out the present study to determine the effect of chikungunya on the collagen and connective tissue metabolism in 75 chikungunya-affected people. First, we screened for mucopolysaccharides in urine by Cetyl Trimethyl Ammonium Bromide (CTAB) test. Appearance of heavy precipitate indicates the presence of higher levels of mucopolysaccharides and later quantified by DMB dye method. The urinary mucopolysaccharide in CHIK patients was 342 ± 45 mg/l compared to healthy controls (45 ± 5.6 mg/l). The collagen building blocks, proline and hydroxyproline were also measured in CHIK patients and observed higher excretion compared to healthy controls. Urinary excretions hydroxyproline was greater than the proline levels. Conclusion These results indicate that CHIK virus infection affects and damage the cartilage and connective metabolism and releases the degraded products from the tissue and responsible for increasing the levels of proline, hydroxyproline and mucopolysaccharides in CHIK affected patients.

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Publié par	biomed
Publié le	01 janvier 2008
Nombre de lectures	5
Langue	English

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Virology Journal

BioMedCentral

Open Access Research Connective tissue metabolism in chikungunya patients 1 21 Sudarsanareddy Lokireddy, Sarojamma Vemulaand Ramakrishna Vadde*

1 2 Address: Departmentof Biotechnology, Sri Krishnadevaraya University, Anantapur, India andDepartment of Community Medicine, Government Medical College, Anantapur, India Email: Sudarsanareddy Lokireddy  l_suda2003@yahoo.co.in; Sarojamma Vemula  vemula_saroja@yahoo.co.in; Ramakrishna Vadde*  krishnavr2007@rediffmail.com * Corresponding author

Published: 27 February 2008Received: 16 January 2008 Accepted: 27 February 2008 Virology Journal2008,5:31 doi:10.1186/1743-422X-5-31 This article is available from: http://www.virologyj.com/content/5/1/31 © 2008 Lokireddy et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Chikungunya (CHIK) fever is a viral disease transmitted to humans by the bite of Chikungunya virus (CHIK virus) infectedAedesmosquitoes. CHIK virus is a member of the Alphavirusgenus of the familyTogaviridae. Previous reports have indicated that infection with CHIK virus produces an acute arthritis in human hosts by large area of necrosis and collagenosis or fibrosis. Results:We carried out the present study to determine the effect of chikungunya on the collagen and connective tissue metabolism in 75 chikungunya-affected people. First, we screened for mucopolysaccharides in urine by Cetyl Trimethyl Ammonium Bromide (CTAB) test. Appearance of heavy precipitate indicates the presence of higher levels of mucopolysaccharides and later quantified by DMB dye method. The urinary mucopolysaccharide in CHIK patients was 342 ± 45 mg/l compared to healthy controls (45 ± 5.6 mg/l). The collagen building blocks, proline and hydroxyproline were also measured in CHIK patients and observed higher excretion compared to healthy controls. Urinary excretions hydroxyproline was greater than the proline levels. Conclusion:These results indicate that CHIK virus infection affects and damage the cartilage and connective metabolism and releases the degraded products from the tissue and responsible for increasing the levels of proline, hydroxyproline and mucopolysaccharides in CHIK affected patients.

Background Chikungunya (CHIK) fever is a viral disease transmitted to humans by the bite of Chikungunya virus (CHIK virus) infectedAedesmosquitoes. CHIK virus is a member of the Alphavirusgenus of the familyTogaviridae. CHIK virus was first isolated from the serum of a febrile patient during a dengue epidemic that occurred in the Newala District, Tanzania in 1953 [1]. TheAlphavirusesare enveloped par ticles and their genome consists of a singlestranded, pos itivesense RNA molecule of approximately 12000 nucleotides. CHIK virus is an important human pathogen

that causes a disease syndrome characterized by fever, headache, rash, nausea, vomiting, myalgia and arthralgia [28]. Its association with a fatal haemorrhagic condition was reported in India [9]. CHIK virus is geographically distributed from Africa through Southeast Asia, and its transmission to humans is mainly throughAedesspecies mosquitoes [1]. Since 1953, CHIK viurs has caused numerous welldocumented outbreaks and epidemics in both Africa and Southeast Asia, involving hundreds of thousands of people [1012]. Recent reports have described a massive outbreak of CHIK disease occurring

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