Zeatin- and polyamine-induced nitric oxide biosynthesis in Arabidopsis thaliana seedlings and its role in signal transduction [Elektronische Ressource] / von Ni Ni Tun Krywen

Zeatin- and Polyamine-Induced Nitric Oxide Biosynthesis inArabidopsis thaliana seedlings and its Role in SignalTransductionVon der Naturwissenschaftlichen Fakultätder Universität Hannoverzur Erlangung des akademischen Grades einerDoktorin der Gartenbauwissenschaften Dr. rer. hort.genehmigte DissertationvonM.Sc.Ni Ni Tun Krywengeb. 25.08.1971 in Min Tut, Myanmar2006______________________________________________________________________________________________________________________________________Referent: Prof. Dr. Günther F. E. SchererKorreferent: Prof. Dr. Manfred SchenkTage der Promotion: 11.05.06iABSTRACTABSTRACTThe effect of zeatin and different polyamines (PAs) on NO (nitric oxide) biosynthesis inArabidopsis seedlings and Nicotiana tabacum cells was examined in this study. Using a cell-impermeable NO-specific fluorescent dye DAR-4M (diaminorhodamine-4M), the release ofNO into the incubation medium was measured. NO production was stimulated by zeatin inboth Arabidopsis wild type and nitrate reductase (NR) deficient nia1 nia2 double mutantseedlings. NO production was dependent on zeatin concentrations and time of incubation.Release of NO was inhibited by the NO scavenger, PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxy-3-oxide), showing that the fluorescence formation was NOspecific.