Vipp1 structure and function in cyanobacteria and chloroplasts [Elektronische Ressource] / vorgelegt von Elena Aseeva

Vipp1 structure and function in cyanobacteria and chloroplasts Dissertation zur Erlangung des Doktorgrades der Fakultät für Biologie der Ludwig-Maximilians-Universität München Vorgelegt von Elena Aseeva München 14.11.2005 Gutachter: 1. Prof. Dr. J. Soll 2. PD Dr. U. Vothknecht Tag der mündlichen Prüfung: 19.12.2005 Contents: Abreviatons iv 1. Abstract 1 2. Zusamenfasung 2 3. Introduction 3 3.1. Evolution of oxygenic photosynthesis 3 3.2. Structure and composition of the thylakoid membrane 4 3.3. Formation of the thylakoid membrane 5 3.4. Vipp1 is an ubiquitous component of thylakoid biogenesis 7 3.5. Phage shock protein A (PspA) of bacteria 9 4. Materials 13 4.1. Chemicals 13 4.2. Enzymes and kits 13 4.3. Primers 4.4. Vectors 14 4.5. E. coli strains 14 4.6. Antibodies 4.7. Other materials 14 4.8. Plant material and growth conditions 15 4.9. Synechocystis growth conditions 15 5. Methods 16 5.1. Molecular biological methods 16 5.1.1. Polymerase Chain Reaction (PCR) 16 5.1.2. Cloning techniques 16 5.2. Biochemical methods 17 5.2.1. Determination of chlorophyll concentration 17 5.2.2. Determprotein 5.2.3. SDS-Polyacrylamid electrophoresis (SDS-PAGE) and Western-blotting 17 5.2.4.