USPL1, a novel SUMO isopeptidase [Elektronische Ressource] / submitted by Lukasz Kozaczkiewicz

USPL1, a novel SUMO isopeptidase PhD Thesis in partial fulfilment of the requirements for the degree “Doctor of Philosophy (PhD)” in the Molecular Biology Program at the Georg August University Göttingen, Faculty of Biology submitted by Lukasz Kozaczkiewicz born in Zakopane, Poland 2009 Affidavit I hereby declare that this doctoral thesis has been written only by the undersigned and without any assistance from third parties. Furthermore, I confirm that no sources have been used in the preparation of this thesis other than those indicated in the thesis itself. thGöttingen, 30 January 2009 Lukasz Kozaczkiewicz Rodzicom List of publications: Book chapter Stankovic-Valentin, N., Kozaczkiewicz, L., Curth, K., Melchior, F. An in vitro FRET-based assay for the analysis of SUMO conjugation and isopeptidase cleavage. Methods Mol Biol. 2009, 497:241-51. TABLE OF CONTENTS Table of Content 1 Acknowledgments 4 Abstract 5 Abbreviations 6 List of Figures 9 List of Tables 10 Chapter 1. Introduction 11 1.1 Protein modification with Ubl 11 1.1.1Ubiquitin-mediated protein degradation 15 1.2 SUMO 16 1.2.1 SUMO modifying enzymes 17 1.2.2 Non covalent SUMO interaction 18 1.2.