Transgenic expression of antimicrobial peptides from insects as a tool for analysis of compatibility between plants and pathogens [Elektronische Ressource] / vorgelegt von Walaa Said Mohamed Shaaban Khalifa

Transgenic expression of antimicrobial peptides frominsects as a tool for analysis of compatibilitybetween plants and pathogensDissertation zur Erlangung des Doktorgrades(Doktor der Agrarwissenschaften)Agrarwissenschaften, Ökotrophologie und Umweltmanagementder Justus-Liebig-Universität Gießendurchgeführt amInstitut für Phytopathologie und Angewandte Zoologievorgelegt vonM. Sc. Walaa Said Mohamed Shaaban Khalifaaus ÄgyptenGiessen 2010Dekanin: Prof. Dr. Ingrid-Ute Leonhäuser1. Gutachter: Prof. Dr. Karl-Heinz Kogel2. Gutachter: Prof. Dr. Andreas VilcinskasBoard of ExaminersChairman of the Committee: Prof. Dr. Günter Leithold1. Referee: Prof. Dr. Karl-Heinz Kogel2. Referee: Prof. Dr. Andreas Vilcinskas3. Examiner: Prof. Dr. Sylvia Schnell4. Examiner: Prof. Dr. Uwe WenzelDate of oral examination: 11.05.2010To my father in spirit whom I always remember, and my dear mother forher love and to my husband who helped me to finish this work and finallyto my son Ziad that I wish him a good future.CONTENTSContentsPage1 Introduction…………………………………………..…………………………… 11.1 Antimicrobial peptides (AMPs)……………………...…………………………. 11.2 AMPs from insects……………………………………………………………… 31.2.1 Insect defensins………………………………………...…………………… 41.2.1.1 Eristalis defensin…………………………………...…………………... 51.2.2 Thanatin……..…………………………………………….……………….. 61.3 Mode of action of AMPs…..…………… 81.4 Production of recombinant AMPs through bacterial expression systems………. 131.