Tracing the editing history of a single B-lymphocyte [Elektronische Ressource] / submitted by Tobias Gerdes

Tracing the editing history of a single B lymphocyte Dissertation in Biology Institut für Anthropologie und Humangenetik der Ludwig-Maximilians-Universität München in collaboration with Department of Microbiology and Immunology University of California, San Francisco Submitted by Tobias Gerdes The dissertation was carried out in Prof. Matthias Wabl’s laboratory at the University of California, San Francisco. The following articles were published based on this work: Gerdes, T., and Wabl, M. (2002). Physical map of the mouse lambda light chain and related loci. Immunogenetics 54, 62-65. Gerdes, T., and Wabl, M. (2004). Autoreactivity and allelic inclusion in a B cell nuclear transfer mouse. Nat Immunol 5, 1282-1287. Supervisors: Prof. Dr. Elisabeth Weiss Prof. Dr. Dirk Eick External supervisor: Prof. Dr. Matthias Wabl thDissertation was submitted Juli 12 , 2006 Table of content 1 1. SUMMARY 3 2. INTRODUCTION 5 2.1 The dogma of clonal selection 5 2.2 Central B-cell development 5 2.2.1 H-chain rearrangement 5 2.2.2 L-chain rearrangement 8 2.2.3 Allelic and isotypic exclusion 9 2.3 Peripheral B-cell development 10 2.3.1 Transitional B cells 10 2.3.2 Autoreactivity 13 2.3.3 Editing 14 2.3.4 Mature B cells 17 2.4 Autoreactivity, editing and allelic inclusion 17 2.5 The nuclear transfer mouse 18 3. SPECIFIC AIMS 21 4.