TopSpot vario [Elektronische Ressource] : a novel microarrayer system for highly parallel picoliter dispensing / Chris Steinert

Chris SteinertTopSpot Vario: A Novel Microarrayer System for Highly Parallel Picoliter DispensingDissertationzur Erlangung des Doktorgrades der Fakultät für Angewandte Wissenschaften der Al-bert-Ludwigs-Universität Freiburg im BreisgauFreiburg i. Br., Juni 2005DekanProf. Dr. Jan G. KorvinkReferentenProf. Dr. Roland Zengerle (Freiburg). Jan G. Korvink (Freiburg)Tag der Prüfung16.01.2006Institut für Mikrosystemtechnik (IMTEK)Lehrstuhl für AnwendungsentwicklungFakultät für Angewandte WissenschaftenAlbert-Ludwigs-Universität FreiburgDISSERTATION CHRIS STEINERT ABSTRACTABSTRACTThis thesis reports about the conceptional design, development and characterizationof a novel system for production of microarrays, a so called microarrayer system. Inthe recent years microarray technology had a high impact in basic research, diagnosticsand drug discovery. Its success caused tremendous activity of different companies toprovide the most suitable microarrayer system. The here presented microarrayer baseson the commercially available TopSpot technology and is called TopSpot Vario. TheTopSpot technology is an alternative to the piezo tip and pin printers which are current-ly dominating the microarrayer market. Its main advantage is the parallel ejection ofup to 96 different samples and its one to one format change from the microtiterplateordered reservoir pattern to the microarray spot pattern.