The translocase of the outer membrane of mitochondria (TOM complex) [Elektronische Ressource] : recognition of mitochondrial targeting signals / von Tincuta Stan

The Translocase of the Outer Membrane of Mitochondria (TOM Complex): Recognition of Mitochondrial Targeting Signals Dissertation zur Erlangung des Doktorgrades des Fachbereischs für Biologie der Ludwig-Maximilians-Universität München von Tincuta Stan aus Galati/Rumänien München 2003 Dissertation eingereicht am 10. 07. 2003 Tag der mündlichen Prüfung: 22. 10. 2003 Erstgutachter: Prof. Dr. R. G. Herrmann Zweitgutachter: Prof. Dr. J. Soll Sondervotum: Prof. Dr. Dr. W. Neupert 2 CONTENTS 1. INTRODUCTION 11.1. Origin, structure and function of mitochondria 1 1.2. Preprotein import into mitochondria 2 1.3. Mitochondrial targeting signals 6 1.4. The TOM complex 71.5. BCS1 protein 101.6. Aims of the present study 12 2. MATERIAL AND METHODS 13 2.1. Molecular Biology Methods 3 2.1.1. Small and large scale preparation of plasmid DNA from E. coli 13 2.1.2. Preparation of yeast DNA 14 2.1.3. Polymerase Chain Reaction 14 2.1.4. Enzymatic manipulation of DNA 14 2.1.5. Preparation and transformation of competent cells 15 2.1.6. DNA purification and analysis 16 2.1.7. Cloning 16 2.2. Genetic Methods 20 2.2.1. E. coli 2 2.2.2. N. crassa 02.2.3. S. cerevisiae 1 2.3.