The structure of human procathepsin S [Elektronische Ressource] : crystallographic investigations on the functional role of the propeptide / von Guido Kaulmann

The Structure of Human Procathepsin S:Crystallographic investigations on the functional role of the pr opeptideDissertationzur Erlangung des akademischen GradesDoc tor rerum na turalium ( Dr . rer. nat.)V orgelegt demR at der Biologi sch- Phar mazeut ischen Fakul tätder Friedrich- Schiller-U ni ver sität Jenavon D ipl. B iol. Gui do K aul manngebor en am 20.01.1966in Bielef eldGutachter :1. Prof. Dr. Bernd Wi ederanders2. Prof. Dr. Rolf Hilgenfeld3. Prof. Dr. D ieter Brőmme--------------------------------------------------------C ontentsContents PageZusammenfassung 1 1. Introduction 41.1 Cathepsin S : tissue distribution and function 41.2 Synthesis, transport an d activation 51.3 Structure of cathepsin S 61.4 The propeptide of cathepsin S 61.4.1 Primary s truc ture and hom ology 61.4.2 The prope ptide as inhi bitor 81.4.3 Folding c ompetenc e 81.4.4 The ER(F/W )N(I/ V )N-m otif and t he hydrophobi c core 91.5 Aim of th e thesis 102. Materials 112.1 Molecular biology 112.1.1 V ectors and pl asmids 112.1.2 Bacterial stra ins, m edia 112.1.3 Kits 112.1.4 Enzymes and buffers 122.1.5 Oligonuc leotide s 122.2 Bac terial expression 132.2.1 Stra in a nd induc er 132.2.2 Buffers for c ell lysis 132.3 Bac ulovirus expression 132.3.1 Ins ect cell culture 13TM2.3.2 Bac-N-Blue Kit (Invi troge n) 132.3.3 M edia and s upplements 132.3.4 Cul ture equi pment 142.4 SDS-Page and We stern blot 142.4.1 Antibodi es 142.4.