The roles of deiodinases in thyronamine biology [Elektronische Ressource] / von Susanne Piehl, geb. Günther

The roles of deiodinases in thyronamine biology D i s s e r t a t i o n zur Erlangung des akademischen Grades d o c t o r r e r u m n a t u r a l i u m (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Dipl.-Humanbiologin Susanne Piehl, geb. Günther Geb. am 26.11.1980 in Wolfen Präsident der Humboldt-Universität zu Berlin Prof. Dr. Dr. h.c. Christoph Markschies Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. Christian Limberg Gutachter/ innen: 1 Prof. Dr. Werner Kloas 2 Prof. Dr. Josef Köhrle 3 Prof. Dr. Gudrun Brockmann Tag der mündlichen Prüfung: 03. Juli 2008 Abstract 3-iodothyronamine (3-T AM) and thyronamine (T AM) are novel endogenous signaling molecules 1 0that exhibit great structural similarity to thyroid hormones but apparently antagonize classical thy-roid hormone (T ) actions. The present study investigated whether thyronamines (TAMs) are sub-3strates of the three Dio isozymes (Dio1, Dio2 and Dio3). TAMs were incubated with isozyme specific Dio preparations. Deiodination products were ana-lyzed using a newly established method applying liquid chromatography and tandem mass spec-trometry (LC-MS/MS).