The role of the RNA-binding protein Hfq in the model pathogen Salmonella typhimurium [Elektronische Ressource] / vorgelegt von Alexandra Sittka

The role of the RNA-binding protein Hfq in the modelpathogen Salmonella TyphimuriumDissertationzurErlangung des Doktorgradesder Naturwissenschaften(Dr. rer. nat.)dem FachbereichPharmazeutische Chemieder Philipps-Universität Marburgvorgelegt vonAlexandra Sittkaaus BerlinBerlin 2008Vom Fachbereich Pharmazeutische Chemie der Philipps-Universität Marburgals Dissertation am______________________________angenommen.Erstgutachter Prof. Dr. Roland K. HartmannZweitgutachter Prof. Dr. Annegret WildeTag der mündlichen Prüfung 19. November 2008Table of contents ITable of contents I-IV1. Introduction 11.1 Sm and Sm-like proteins 11.2 The Sm-like protein Hfq 21.3 RNA-binding properties of the Lsm protein, Hfq 31.4 Hfq and its pleiotropic binding partners in bacteria 61.5 Physiological role of Hfq in bacteria 81.6 Post-transcriptional gene regulation in bacteria 91.6.1 Repression of translation via sRNAs 101.6.2 Activation of translation via sRNAs 121.7 Influence of Hfq in post-transcriptional gene regulation 141.8 The model pathogen Salmonella enterica Serovar Typhimurium 161.9 References 182. Goal of the project 263. Methods 283.1 Bacterial cell culture 283.1.1 Media 283.1.2 Preparation of electrocompetent Salmonella cells 293.1.3 Transformation of chemically competent E. coli cells 293.1.4 Growth under SPI-1 inducing conditions 293.1.5 Growth under SPI-2 inducing conditions 303.1.6 Growth curves 313.1.7 Motility assay 313.