The role of the plasmalemmal ATP-sensitive potassium channels (K_1tnA_1tnT_1tnP) in induction of endothelial ischemia-reperfusion injury [Elektronische Ressource] / by Dragan Gligorievski

1 The role of the plasmalemmal ATP-sensitive potassium channels (K ) in induction ATPof endothelial ischemia-reperfusion injury Inaugural Dissertation submitted to the Faculty of Medicine in partial fulfillment of the requirements for the PhD-Degree of the Faculties of Veterinary Medicine and Medicine of the Justus Liebig University Giessen by Dragan Gligorievski from Kumanovo, Republic of Macedonia Giessen 2007 2 From the Institute of Physiology Chairman: Prof. Dr. Dr. Hans-Michael Piper of The Faculty of Medicine of the Justus Liebig University Giessen First Supervisor and Committee Member: Prof. Dr. Dr. Hans-Michael Piper Second Supervisor: Prof. Dr. Ulrich Decking Committee Member: Prof. Dr. Heinz-Jürgen Thiel Commber: Prof. Dr. Martin Diener Date of Doctoral Defense: thJune 27 , 2007 3Contents 1 Introduction 1 1.1 Endothelium and its barrier function 2+1.2 The role of Ca in microvascular endothelial cells during simulated ischemia and reperfusion 2 2+1.3 The role of K channels in modulation of Ca influx during ATP ischem 2 1.4 Molecular structure of K channels and their role ATPin ischemia and reperfusion 4 1.5 Aim of the study 5 2 Materials 6 2.1 Chemicals 6 2.2 Buffers 8 2.2.