The role of hepatic cholesterol transporter ABCA1 for HDL metabolism in vivo [Elektronische Ressource] : an adenovirus-mediated RNA interference approach in mice (Mus musculus, Linnaeus 1758) / vorgelegt von Sergey Ragozin

The role of hepatic cholesterol transporter ABCA1 for HDL metabolism in vivo An adenovirus-mediated RNA interference approach in mice (Mus musculus, Linnaeus 1758) Dissertation zur Erlangung des Doktorgrades des Fachbereichs Biologie der Universität Hamburg vorgelegt von Sergey Ragozin aus Orenburg, Russland Hamburg 2004 Моей маме с нежностью и любовью Felix, qui potuit rerum cognoscere causas Table of contents 1. Introduction 1 1.1. Lipoprotein metabolism 1 Lipoproteins 1 Exogenous metabolic pathway 3 Endogenous me 4 Reverse cholesterol transport 5 1.2. The role of ABCA1 transporter for HDL metabolism 7 Family of transmembrane transporters 7 ABCA1 – major transporter for cholesterol and phospholipids 9 Tanger Disea 12 Animal models for studying ABCA1 in HDL metabolism 12 1.3. Post-transcriptional gene silencing – RNA interference 14 Phenomenon and mechanism of RNA interference in vertebrates 15 Stable expression of the small interfering RNAs for functional gene knock-down 17 1.4. Adenoviral vectors and gene delivery 18 Structure and function of human adenovirus serotype 5 19 Recombinant adenovirus for in vitro and in vivo biological studies 20 1.5. The aim of the study 22 2. Methodological considerations 23 2.1.