The role of Epstein-Barr virus nuclear antigen 3C in the immortalisation process of primary human B-lymphocytes [Elektronische Ressource] / von Madelaine Löfqvist

The role of Epstein-Barr virus nuclear antigen 3C in the immortalisation process of human primary B-lymphocytes Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München von Madelaine Löfqvist München, Februar 2004 First examiner: Prof. Dr. D. Eick Second examiner: Prof. Dr. J. Parsch Date of exam: 8 Juli 2004 Table of contents 1. Introduction........................................................................................................... 6 1.1 Herpesviruses...............................................................................................................6 1.1.1 The Epstein-Barr virus (EBV) ................................................................................................ 7 1.1.2 Malignancies associated with EBV........................................................................................ 8 1.1.3 EBV genetics using BACs ..................................................................................................... 8 1.2 In vitro infection of primary human B-lymphocytes as a model system for EBV induced B-cell immortalisation .......................................................................................11 1.2.1 Formation of lymphoblastoid cell lines................................................................................. 11 1.2.2 Viral proteins involved in B-cell immortalisation .................................................................. 11 1.