The Repulsive Guidance Molecule-A attenuates chemotaxis induced neutrophil migration and dampens inflammation [Elektronische Ressource] / vorgelegt von Sebastian Matthias Josef Brown

- 1 - Aus der Universitätsklinik für Anästhesiologie und Intensivmedizin Tübingen Ärztlicher Direktor: Professor Dr. K. Unertl The Repulsive Guidance Molecule – A attenuates chemotaxis induced neutrophil migration and dampens inflammation. Inaugural-Dissertation zur Erlangung des Doktorgrades der Medizin der Medizinischen Fakultät der Eberhard Karls Universität zu Tübingen vorgelegt von Sebastian Matthias Josef Brown aus Bonn 2010 - 2 - Dekan: Professor Dr. I. B. Autenrieth 1. Berichterstatter: Professor Dr. P. Rosenberger 2. Berichterstatter: Professor Dr. G. Klein - 3 - - 4 - INDEX 1 Introduction.......................................................................................... 7 1.1 Migration of leukocytes in response to acute inflammation. 7 1.2 Neuronal guidance molecules as immune modulating proteins. 10 1.3 The Repulsive Guidance Molecule A. 11 1.4 Hypothesis and goal of this study. 14 1.5 Support and cooperation concerning this study. 15 2 Materials and Methods ...................................................................... 16 2.1 Materials 16 2.1.1 Transcriptional studies ............................................................ 16 2.1.2 Immunoblotting experiments...................................................