The oxygen sensors FNR from Escherichia coli and NreABC from Staphylococcus carnosus [Elektronische Ressource] / Florian Reinhart

The Oxygen Sensors FNR from Escherichia coli and NreABC from Staphylococcus carnosus Dissertation Zur Erlangung des Grades “Doktor der Naturwissenschaften” Am Fachbereich Biologie der Johannes Gutenberg-Universität Mainz Florian Reinhart geb. am 25.01.1980 in Mainz Mainz, 13.10.2010 Dekan: Prof. Dr. XXX 1. Berichterstatter: Prof. Dr. XXX 2. Berichterstatter: Prof. Dr. XXX Tag der mündlichen Prüfung: November 2010 "Doing what little one can to increase the general stock of knowledge is a respectable object of life." Charles Darwin CONTENTS  Contents 1 Abstract 12 Introduction 22.1 Oxygen sensing by bacteria 22.22.2 FNRFNR,, aa d diirreecctt O O sseensnsoorr f frromom EEssccherheriicchhiia ca coolili 44222.3 The NreBC two-component system from Staphylococcus carnosus 62+2.4 Anerobic NreB features a [4Fe4S] cluster which is required for9kinase activity2.52.5 NNrreeAA, a , a GGAAF dF doommaainin prprootteeiinn wwiitthh uunnkknnoowwnn ffuncunctitionon 11113 Materials & Methods 133.1 Strains and plasmids3.13.1..11 BBacacttereriiaall ssttrraiainnss aanndd p pllasasmmiiddss 13133.1.2 Long-term storage of bacterial strains 143.2 Bacterial growth and media3.23.2..11 GrGroowwtthh o off EEsscchheerriicchihia cola colii 14143.2.3 Growth of Staphylococcus carnosus 143.2.4 Media and buffers for Escherichia coli 153.2.