The impact of biomechanical forces on the differentiation of vascular cells during arteriogenesis [Elektronische Ressource] / presented by Elena Demicheva

DissertationsubmittedtotheCombinedFacultiesfortheNaturalSciencesandforMathematicsoftheRupertoCarolaUniversityofHeidelberg,GermanyforthedegreeofDoctorofNaturalSciences The impact of biomechanical forces on the differentiation of vascular cells during arteriogenesis PresentedbyDiplom–Biochemist ElenaDemichevabornin Moscow,RussianFederationthOralexamination April,28 ,2008 Referees: Prof.Dr.M.Hecker Prof.Dr.P.Angel Prof.Dr.D.Robinson PDDr.M.Mayer2 Contents Abbreviations 8 1. Introduction 10 1.1. Clinical relevance of arteriogenesis vs. angiogenesis 10 1.2. Physical forces regulating arteriogenesis 15 1.3. Molecular mechanisms of arteriogenesis 16 1.3.1. Chemokinesandarteriogenesis 18 1.3.2. TranscriptionalregulationofMCP1 19 1.4. Aims of the study 20 2. Materials 21 2.1. Antibodies 21 2.2. Cell culture 21 2.3. Bacteria and plasmid vectors 22 2.4. Primers 23 2.5. Decoy oligodeoxynucleotides 23 2.6. Kits 24 2.7. Analysis software and online tools 24 2.8. Buffers and stock solutions 25 2.8.1. Histologicalbuffersandstocksolutions 25 2.8.2. Cellbiologybuffersandstocksolutions 25 2.8.3. Molecularbiologysolutions 26 3 3. Methods 27 3.1. Invivo and insitu models 27 3.1.1. Hindlimbischemiamodel 27 3.1.2.