The generation and characterisation of mice with conditional knock-out of the NMDAR subunit NR2B [Elektronische Ressource] / presented by Beril Doğancı

DISSERTATION submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by MD Beril Do ğancı born in Sakarya, Turkey The Generation and Characterisation of Mice with Conditional Knock-out of the NMDAR Subunit NR2B Referees: Prof. Dr. Peter Seeburg Prof. Dr. Hannah Monyer Dedicated to my grandfather R. Özdemir , my parents Gülnihal and A. Oktay Do ğancı. Acknowledgements Many thanks to: Prof. Dr. Hannah Monyer for interesting project and excellent working conditions. Prof. Dr. Peter H. Seeburg for continuous scientific input, supervision and valuable ideas. Dr. Anne Herb for reading the manuscript and critical remarks. Dr. Jakob von Engelhardt for performing the electrophysiological analysis. Thorsten Schächinger for his work during the generation of the targeting construct. Dr. Miyoko Higuchi for her help in organisation and breeding of the mouse lines and for helpful remarks. Dr. Isabel Aller for continuous moral support, helpful remarks. Dr. Elke Fuchs for introductory help in ES cell work. Dr. Valery Grinevich for constructing and providing the viral construct. Ali Çetin and Dr. Pavel Osten for teaching and helping for the steriotaxy.