The function of Toc34 and its regulation [Elektronische Ressource] / vorgelegt von Marko Jelić

The function of Toc34 and its regulation Inaugural-Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Univerität München vorgelegt von Marko Jeli ć München 2003 In Deo Gloria Nadani, Mariji i Luciji Terezi 2 1. Gutachter: Prof. Dr. Jürgen Soll 2. Gutachter: Prof. Dr. Jürgen Ebel Tag der Mündliche Prüfung: 16. Oktober, 2003. 3CONTENTS ABBREVIATIONS..............................................................................................6 1. ABSTRACT......................................................................................................7 2. ZUSAMMENFASSUNG.................................................................................9 3. INTRODUCTION ......................................................................................... 11 4. MATERIALS AND METHODS................................................................. 19 4.1 General..................................................................................................................... 19 4.2 Generation and overexpression of the preproteins and atToc33∆∆∆∆TM, atToc34∆TM and site-directed mutants...................................................................... 19 4.3 Introduction of point mutations into Toc34 and protein expression..................20 4.4 Immobilisation of Toc33 onto GTP or preSSU affinity matrix ..........................20 4.