Structure function studies of ABC transporters: HlyB from Escherichia coli and ChoX from Sinorhizobium meliloti [Elektronische Ressource] / vorgelegt von Christine Oswald

Structure-function studies of ABC transporters HlyB from Escherichia coli and ChoX from Sinorhizobium meliloti Inaugural-Dissertation zur Erlangung des Doktorgrades der Mathemathisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf vorgelegt von Christine Oswald aus Memmingen Januar 2008 Aus dem Institut für Biochemie der Heinrich-Heine-Universität Düsseldorf Gedruckt mit der Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf Referent: Prof. Dr. L. Schmitt Koreferent: Prof. Dr. G. Groth Tag der mündlichen Prüfung: 23.01.2008 Meinen Eltern Summary ATP binding cassette (ABC) transporters represent a superfamily of ubiquitous membrane proteins which utilize energy derived from binding and hydrolysis of ATP to fuel the translocation of substrates. The transport substrates are highly diverse in their chemical characteristics and their size. Generally, this superfamily can be divided in importers and exporters. In contrast to export systems, archaeal and bacterial importers exhibit an additional substrate binding protein (SBP), which captures the substrate and delivers it to the ABC transporter. Binding of the SBP to the transporter triggers ATP hydrolysis and substrate transport.