Structural requirements and role of oxidoreductase features for {Kvβ-mediated [Kv-beta-mediated] potassium channel inactivation [Elektronische Ressource] / vorgelegt von Vitya Andranic Vardanyan

Institut für Neurale Signalverarbeitung Zentrum für Molekulare Neurobiologie Hamburg Falkenried 94, 20251 Hamburg Structural requirements and role of oxidoreductase features for Kvβ-mediated potassium channel inactivation Dissertation Zur Erlangung des Doktorgrades des Fachbereiches Biologie der Universität Hamburg Vorgelegt von Vitya Andranic VARDANYAN Hamburg 2003 1 TABLE OF CONTENTS 1. Introduction 1.1 Structure of potassium channels.............................................................................................6 1.2 Inactivation of Shaker-related potassium channels................................................................9 1.3 Beta subunits of Shaker-related potassium channels............................................................10 2. Materials and Methods 2.1 Molecular biology.........................................................................................................13 2.1.1 Clones and vectors........................................................................................................13 2.1.2 In vitro mutagenesis......................................................................................................14 2.1.3 Kv1.2/Kv2.1 and Kvβ1.1/Kvβ3 chimeras...................................................................14 2.1.4 In vitro RNA synthesis..............................................