Sterol requirements in Drosophila melanogaster [Elektronische Ressource] / von Maria João Almeida de Carvalho

Sterol requirements in Drosophila melanogaster Dissertation zur Erlangung des akademishen Grades Doctor rerum naturalium (Dr. rer. nat.) vorgelegt der Fakultät Mathematik and Naturwissenschaften der Technischen Universität Dresden von Maria João Almeida de Carvalho geboren am 10 Februar 1981 in Coimbra, Portugal Gutachter: Prof. Dr. Elisabeth Knust, Technisch Universität Dresden Prof. Dr. med. Michele Solimena, Technisch Universität Dresden Eingereicht am: 11.06.2009 Verteidigt am: 28.09.2009 To my parents… PLEASURES The first look out of the window at dawn The old book, found again Joyful faces Snow, the change of seasons The newspaper The dog The dialectics Taking a shower, going for a swim Old music Comfy shoes Comprehension New music Writing, gardening Traveling Singing Being friendly. Bertolt Brecht (1898-1956) TABLE OF CONTENTS Page SUMMARY 1 I. INTRODUCTION 2 I-1. Lipids 5 I-1.1. Glycerophospholipids 6 I-1.2. Sphingolipids 7 I-1.3. Sterol Lipids 8 I-1.3.1. Sterol lipids in prokaryotes and in invertebrates 11 I-1.3.1.1. Sterol lipids in Drosophila melanogaster 13 I-2. Development of Drosophila melanogaster 17 II. SCOPE OF THE THESIS 20 III. RESULTS 23 III-1. Lipid-depleted medium and the feeding experiments 24 III-2.