Spectroscopic studies of ligand migration in myoglobin [Elektronische Ressource] / Karin Nienhaus

Spectroscopic Studies of Ligand Migration in Myoglobin Dissertation zur Erlangung des Doktorgrades Dr. rer. nat. der Fakultät für Naturwissenschaften der Universität Ulm vorgelegt von Karin Nienhaus Rhede 2002 Amtierender Dekan: Prof. Dr. Wolfgang Witschel Erstgutachter: Prof. Dr. Peter Bäuerle Zweitgutachter: Prof. Dr. Hartmut Seliger Tag der Promotion: 13.02.2003 Contents: 1. Introduction ............................................................................................................. 1 1.1 Protein Structure, Function, Dynamics ............................................................. 1 1.2 Myoglobin, the Hydrogen Atom of Biology..................... 3 Brief Historical Overview ................................................................................. 3 1.2.1 Structure and Function.......... 3 1.2.2 Ligand Binding and Protein Dynamics................. 5 1.2.3 Infrared Spectroscopy............................................................................ 13 1.2.4 Outline of this work............... 17 2. Materials and Methods ............................................................................................ 19 2.1 Protein Expression and Purification.................................. 19 2.1.1 Transformation and Multiplication........................ 19 2.1.