Spectroscopic and Functional Characterization of the Heme Proteins Nitrophorin 4, Nitrophorin 7, and Selected Site-Directed Mutants: Studies of Their Nitrite Reactivity and of the Ferroheme Forms [Elektronische Ressource] / Chunmao He

Spectroscopic and Functional Characterization of the Heme Proteins Nitrophorin 4, Nitrophorin 7, and Se-lected Site-Directed Mutants: Studies of Their Nitrite Re-activity and of the Ferroheme Forms Inaugural-Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf vorgelegt von Chunmao He aus Chenzhou, P.R.ChinaMülheim an der Ruhr, December 2011 Aus dem Max-Planck-Institut für Bioanorganische Chemie, Mülheim an der Ruhr Gedruckt mit der Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf Referent: Prof. Dr. Wolfgang Lubitz Koreferent: Prof. Dr. Henrike Heise Tag der mündlichen Prüfung: 13.12.2011 Acknowledgements The work presented here would not have been possible without the enormous support from the following people. First of all, I would like to thank Professor Dr. Wolfgang Lubitz for his kind support of this project and being my supervisor. Professor Dr. Henrike Heise is also acknowledged for being the co-supervisor of my PhD study, and her critically reading of this thesis. Dr. Markus Knipp, who guided me through the whole project, is such a nice tutor show-ing me not only how experiments are running, but also how interesting it is to run the ex-periments. His broad scientific background and attitudes towards science have great in-fluence on this work and also on me in developing my way of doing science.