Specification of the orthogonal axes in Xenopus laevis: Maternal and zygotic genes involved in patterning the dorsal axis [Elektronische Ressource] / Petra D. Pandur

Universität UlmAbt. BiochemieLeiter Prof. Dr. Dr. W. KnöchelAG Prof. Dr. D WedlichAlbert-Einstein-Allee 1189081 UlmSpecification of the Orthogonal Axesin Xenopus laevis:Maternal and Zygotic Genes Involved in Patterning the Dorsal AxisDissertationfor attaining the degreeDoctor biologiae humanae(Doktorgrad der Humanbiologie)at the Medical Faculty of theUniversity of Ulmpresented byPetra D. Pandurfrom Heidenheim2000Dean: Professor Dr. Peter GierschikExpert 1: Professor Dr. Doris WedlichExpert 2: Professor Sally A. Moody, PhDDate of defense: 2 June 2000ContentsAbbreviations V1 Introduction 11.1 Part I: Formation of the dorsal-ventral axis 11.1.1 Molecular asymmetry in the Xenopus embryo 41.2 Part II: Six homeobox transcription factors and their role in head patterning 71.2.1 Molecular structure and functional domains of Six genes 91.2.2 Expression and function of Six genes 102 Rationale of experiments 143 Material and Methods 163.1 Reagents 163.1.1 Kits 183.1.2 Molecular Weight Markers 183.1.3 Radioactive Material 183.1.4 Bacteria 193.1.5 Plasmids 193.1.6 Enzymes 193.1.7 Equipment 193.1.8 Antibodies and other Biologic Reagents 203.1.9 Sequencing 203.2 Methods 213.2.1 Manipulation and Maintenance of Embryos and Explanted Tissues 213.2.1.1 Buffers and Solutions 21I3.2.1.2 Obtaining Embryos by Natural Fertilization 213.