Spatio-temporal control of {Wnt-β-catenin [Wnt-beta-catenin] signaling during fate commitment of human neural progenitor cells [Elektronische Ressource] / vorgelegt von: Benjamin Bader

Dissertationzur Erlangung des akademischen Gradesdoctor rerum naturalium (Dr. rer. nat.)¨der Mathematisch-Naturwissenschaftlichen Fakultatder Universitat¨ RostockSpatio-temporal control of Wnt/β-catenin signaling duringfate commitment of human neural progenitor cellsVorgelegt von:Benjamin M. Badergeb. in HagenowEingereicht am:8. April 2010, RostockBegutachtet von:Prof. Dr. Dieter G. Weiss, Universitat¨ Rostock (Rostock, Deutschland)Prof. Dr. Ernest Arenas, Karolinska Institute (Stockholm, Schweden)Verteidigt im wissenschaftlichen Kolloqium am:7. Juni 2010, Rostockurn:nbn:de:gbv:28-diss2010-0135-6”When you employ the microscope, shake offall prejudice, nor harbour any favouriteopinions; for, if you do, ’tis not unlikelyfancy will betray you into error, and makeyou see what you wish to see.”Henry Baker (1743), Cautions in viewing objectspicture on the previous page: human neural progenitor cells ReNcell VM after three days of differentiation. β-catenin (green), Tuj3(green, neuronal marker), nuclei (blue). 3D-rendering projection using Imaris-software, boxes represent isolated objects after surface-renderingof the nuclear staining which allows the quantification of fluorescence signals in 3D; Single cell: isolated neuron from larger image, 3D-surfacerendering of different sections of a neurite. numbers correspond to the β-catenin fluorescence intensities in each section.Contents IContentsAbbreviations IVSummary VI1 Introduction 11.