Sonic hedgehog signaling pathway in normal and adenomatous pituitary [Elektronische Ressource] / submitted by Greisa Vila

From: Max Planck Institute of PsychiatryDirector: Professor Dr. Dr. F. HolsboerSonic hedgehog signaling pathway in normaland adenomatous pituitaryThesis for the attainment of the title Medical Doctorfrom the Faculty of Medicine, Ludwig-Maximilians-University, MunichSubmitted by Greisa Vila Tirana2004Mit Genehmigung der Medizinischen Fakultätder Universität MünchenBerichterstatter: Prof. Dr. G. K. StallaMitberichterstatter: Prof. Dr. A. KönigProf. Dr. J. HermsMitbetreuung durch den Promovierten Mitarbeiter: Dr. rer. nat. M. Paez-PeredaDekan: Prof. Dr. med. Dr. h. c. K. PeterTag der mündlichen Prüfung: 18.03.20042To Fiona3Table of Contents pageAbbreviations 61. Introduction 81 .1 The pituitary gland and pituitary tumors 81.1.1 The pituitary gland 81.1.2 Regulation of corticotroph cells by CRH 111.1.3 Pituitary tumors 11 1 .2 Sonic hedgehog signal transduction pathway – an overview 12 1 .3 Sonic hedgehog signaling pathway in human disease 16 1.4 Shh signaling in pituitary gland development 182. Aim of the work 203. Methods 213.1 Equipment 213.2 Reagents 223.3 Solutions 243.4 Human tissues 253.5 Immunohistochemistry 253.5.1 Single immunohistochemistry 283.5.2 Double immunohistochemistry 293.6 Cell culture 303.6.1 Rat pituitary primary cell culture 303.6.2 Immortalized pituitary cell lines 313.6.3 Stimulations 313.7 Hormone measurement by RIA 333.