Soft lithography applied to the construction of a low cost bioassay system [Elektronische Ressource] / vorgelegt von Sebastian Lange

Soft Lithography applied to the construction of a low‐cost bioassay systemDISSERTATIONzur Erlangung des Grades eines Doktorsder Naturwissenschaftender Fakultät für Mathematik und Physikder Eberhard‐Karls‐Universität zu Tübingenvorgelegt vonSEBASTIAN LANGEaus Tübingen2004 Tag der mündlichen Prüfung: 26.11.2004Dekan: Professor Dr. P. Schmid1. Berichterstatter:Professor Dr. D. Kern2. Berichterstatter:Professor Dr. S. Dübel3. Berichterstatter:Professor Dr. J. WachtveitelAbstractThe importance of simple, fast, and inexpensive analysis tools for diagnostic marker molecules inpatients’ sera will increase in the near future. Today’s analysis devices for immuno diagnostics orDNA tests are by far too expensive and are thus rarely found in doctors’ offices and hospitals. Fur-thermore, the disposables and carriers for the biotests are fabricated in complicated and cost-inten-sive processes.It was the goal of this work to setup an analysis device for the detection of molecular binding eventsemploying a CD-pickup from conventional CD players. Additionally, simple and fast methods forthe structured immobilization of biomolecules such as proteins or DNA were created, evaluated andimproved.These developments aim at establishing a low-cost platform technology for the analysis of patients’sera, which includes a fabrication method for affordable test carriers such that they could be used ona routine basis in doctors’ offices and hospitals.