Self-assembly of the S-layer protein of Sporosarcina ureae ATCC 13881 [Elektronische Ressource] / von Melinda Varga

SELF-ASSEMBLY OF THE S-LAYER PROTEIN OF SPOROSARCINA UREAE ATCC 13881 Dissertation zur Erlangung des akademischen Grades Doctor rerum naturalium (Dr. rer. nat.) vorgelegt der Fakultät Mathematik und Naturwissenschaften der Technischen Universität Dresden von Melinda Varga (M.Sc. Mol. Bioeng.) geboren am 27.01.1982 in Cluj, Rumänien Gutachter: Prof. Dr. Gerhard Rödel Prof. Dr. Wolfgang Pompe Eingereicht am 29.10.2010 Verteidigt am 24.01.2011 To my parents… „Ever tried. Ever failed. No matter. Try again. Fail again. Fail better. “ Samuel Beckett 2 CONTENTS List of figures and tables 6 Abbreviations 8 1 Introduction 9 1.1 Molecular self-assembly and self-assetembmsl ing sys 10 1.2 Protein crystallization 11 1.2.1 Driving force of protein crystallrmiozdaytniaomni-c athle aspects 11 1.2.2 Protein crystal nucleation 12 1.2.3 Protein crystal growth 16 1.3 Surface layer proteins (S-layers) 17 1.3.1 Structure of S-layers 17 1.3.2 Properties of S-layers 19 1.3.3 Genetics of S-layers 22 1.3.4 Functions of S-layers 23 1.3.5 Genetic engineering of S-layers 24 1.3.6 Applications of S-layers 25 1.3.