The following is a draft of a mini-tutorial we plan to place on the MGC Website to assist MGC clone

A Guide to Finding Mammalian Gene Collection (MGC) Clones and Evaluating Their Sequence Part A. MGC Clone Search. A variety of ways exist to determine whether MGC cDNA 1clones are available for human, mouse, and rat genes and transcripts of interest . Here we describe three approaches. We illustrate by searching for MGC cDNA clones for protein-coding transcripts from the human gene SERPINA1, encoding α-1-anti-trypsin protein. (As per HUGO-defined convention, all letters of human gene names are capitalized, but only the first letter of mouse and rat gene names is capitalized. Entries into the search engines described below are case insensitive.) Approach 1. The MGC homepage (Figure 1) provides several search tools. You can search for individual full-protein coding (full-cds) human, mouse, rat, or bovine clones from this page using gene names or key words. Entering SERPINA1 into the Enter Gene Symbol box (Figure 1, arrow 1) opens a page that shows two MGC clones are available, BC011991 and BC015642 (Figure 2), together with names of the libraries from which they were isolated and links to associated vector and source tissue information. Figure 1 MGC Website Home Page12 1 All newly isolated MGC, XGC, and ZGC clones are assigned a “BC” accession when their sequence is submitted to GenBank, but only a subset of these candidate clones has a full-CDS. Once a candidate clone is confirmed by ...