9-6-06 Seminar Tutorial

Information Relating to “FromHolliday Junctions to HalogenBonds”: A Seminar by Dr. PuiShing Ho9-6-06Nathan AlexanderHolliday Junctions• The point of crossing over for twohomologous recombinating DNA strandsMathews, C., van Hold, K.E., Ahern, K. Biochemistry, 3. Benjamin/Cummings: San Francisco, 2000.DNA• Contains nucleic acid molecules cytosine(C), thymine (T), adenine (A), guanine (G)– A pairs with T– C pairs with G• Can adopt different conformations– B-DNA: stereotypical helix– A-DNA: slightly distorted helix• DNA Sequence: a series of nucleic acidsex. CTACGGCA• Brandt F. Eichman, Jeffery M. Varagason, Blaine H.M. Mooers, and P. Shing Ho (2000) “TheHolliday junction in an inverted repeat DNA sequence: Sequence effects on the structure of four-wayjunctions”, Proc. Natl. Acad. Sci., USA, 97, 3971-3976.Recombination• The mixing of separate DNA to form newDNAAllows genetic variation and mixingMathews, C., van Hold, K.E., Ahern, K. Biochemistry, 3. Benjamin/Cummings: San Francisco, 2000.Homologous DNA• DNA strands that carry genetic informationat the same locus.Mathews, C., van Hold, K.E., Ahern, K. Biochemistry, 3. Benjamin/Cummings: San Francisco, 2000.Holliday Junctions• The point of crossing over for twohomologous recombinating DNA strandsMathews, C., van Hold, K.E., Ahern, K. Biochemistry, 3. Benjamin/Cummings: San Francisco, 2000.If these are two double-helix lengths of homologousDNAThen these are two double-helix lengths ...