Safety testing of indocyanine green and trypan blue on retinal pigment epithelium cells [Elektronische Ressource] / vorgelegt von Andreas Altvater

Aus dem Department für Augenheilkunde TübingenUniversitäts-AugenklinikSchwerpunkt: Erkrankungen des vorderen und hinteren AugenabschnittesÄrztlicher Direktor: Professor Dr. K.-U. Bartz-SchmidtSafety testing of indocyanine green andtrypan blue on retinal pigment epitheliumcellsInaugural-Dissertationzur Erlangung des Doktorgradesder Medizinder Medizinischen Fakultätder Eberhard Karls Universitätzu Tübingenvorgelegt von Andreas AltvaterausSathmar / Rumänien2007Dekan: Professor Dr. I. B. Autenrieth1. Berichterstatter: Professor Dr. S. Grisanti2. Berichterstatter: Professor Dr. H. HoeraufAbstractSafety testing of indocyanine green and trypanblue on retinal pigment epithelium cells.AbstractBackground and aims: Indocyanine green (ICG) and Trypan blue (TB) arefrequently used vital stains for ILM-staining during macular hole surgery. Lately,there are growing concerns in terms of safety to retinal tissues, especially to theretinal pigment epithelium (RPE). The aim of this experimental study was toexamine potentially cytotoxic effects of ICG and TB on cultured human RPEcells.Methods: ARPE-19 cels were incubated with ICG (0.025–5.0 mg/ml) and withICG-free solutions of corresponding osmolarities. TB was applied at 0.0375mg/ml and 1.5 mg/ml. Incubation lasted 1–20 minutes with or without vitrectomyendolight ilumination for 1–5 minutes. To mimic clinical practice, exposure timewas set at 1 minute, followed by 5 minutes of illumination.