Role of cytokines for NK cell competence and differentiation [Elektronische Ressource] / von Kerstin Jülke

Dissertation Role of Cytokines for NK cell Competence and Differentiation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin im Fach Biologie von Diplom-Biochemikerin Kerstin Jülke Präsident der Humboldt-Universität zu Berlin Prof. Dr. Dr. h.c. Christoph Markschies Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. Lutz-Helmut Schön Gutachter: 1. Prof. Andreas Radbruch 2. Prof. Alf Hamann 3. Prof. Hans-Dieter Volk Datum der Promotion: 28.06.2010Table of contents Table of Contents Table of Contents 1Introduction 11.1 The Immune System – Innate and Adaptive Immunity 11.1.1 The innate immune system 11.1.2 The adaptive immune system 21.2 General aspects of NK cell biology and activation 31.3 NK cells subsets and differentiation 61.3.1 NK receptors 71.3.2 Cytokine and chemokine receptors and adhesion molecules 81.3.3 Functional responses 91.4 Mechanism of NK cell self tolerance 101.5 NK subset development 14dim1.5.1 Heterogeneity of CD56 NK cells 162 Aims of the Thesis 173 Materials and Methods 183.1 Buffers, Medias and solutions 183.2 Devices and materials 193.3Chemicals 203.4 Cell culture conditions 203.5 Cell isolation and cytometric analysis 213.5.1 Isolation of Peripheral blood mononuclear cells (PBMC) 21®3.5.