Reverse genetics of PsaA and PsaB to dissect their function in binding and electron transfer from plastocyanin or cytochrome c_1tn6 to the core of photosystem 1 [Elektronische Ressource] / von Frederik Sommer

Reverse genetics of PsaA and PsaB to dissect their function in binding and electron transfer from plastocyanin or cytochrome c to 6the core of photosystem 1 Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) vorgelegt dem Rat der Biologisch-Pharmazeutischen Fakultät der Friedrich-Schiller- Universität Jena von Dipl. Chem. Frederik Sommer geboren am 18.12.1970 in München Jena, November 2003 1. Gutachter: PD M. Hippler 2. Gutachter: Prof. R. Oelmüller 3. Gutachter: Prof. R. Bock Verteidigungstermin: 16.1.2004 Table of contents 1 Introduction 1 1.1 Energy conversion in photosynthesis 1 1.2 The electron transport in thylakoids 2 1.2.1 Photosystem 2 3 1.2.2 Cytochrome b f complex 3 61.2.3 1 5 1.3 Balancing the power 6 1.3.1 Heterogeneity 6 1.3.2 Reactive oxygen species generation 7 1.3.3 Scavenging mechanisms 7 1.4 Reactions at the donor side of PS1 9 1.4.1 Electron transfer in proteins 9 1.4.2 Reaction mechanism of electron transfer to PS1 10 1.4.3 Electron donors to PS1 11 1.4.4 Structural components of PS1 12 1.4.4.1 PsaF 13 1.4.4.2 PsaN 14 1.4.4.3 Recognition site at the core of PS1 14 1.5 Aim of this work 16 2 Publications 17 2.1 Overview of the included publications 2.2 Sommer, F., Drepper, F. and Hippler, M.