Regeneration and plastid transformation approaches in Arabidopsis thaliana and Rapid-Cycling Brassica rapa [Elektronische Ressource] / Areli Herrera Díaz. Betreuer: Hans-Ulrich Koop

Regeneration and plastid transformation approaches in Arabidopsis thaliana and Rapid-Cycling Brassica rapa Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften der Fakultät für Biologie der Ludwig-Maximilians-Universität München Vorgelegt von Areli Herrera Díaz aus Mexiko München, 2011 1. Gutachter: Prof. Dr. Hans-Ulrich Koop 2. Gutachter: Prof. Dr. Jörg Nickelsen Tag der mündlichen Prüfung: 22. Juli 2011 Contents CONTENTS 1 ZUSAMMENFASSUNG ............................................................................ 3 2 SUMMARY .......................... 5 3 INTRODUCTION .................. 7 3.1 Plastids ...................... 7 3.1.1 Plastid division ........................................................................ 8 3.1.2 Plastid inheritance ... 8 3.1.3 Plastid genome ...... 10 3.1.4 Plastid gene expression .......................................................... 11 3.2 Genetic transformation of plastids in higher plants ................. 12 3.2.1 Gene transfer methods and transformation vectors .................... 12 3.2.2 Selection markers .................................. 13 3.2.3 Reporter genes ...................................... 16 3.2.4 Plastid transformation in Nicotiana tabacum .............................. 16 3.2.5 Plastid transformation in different species . 18 3.3 Arabidopsis thaliana as model organism . 18 3.3.