RBP-J dependent and independent signalling of EBNA-2 [Elektronische Ressource] / Kristina Grabušić

RBP-J DEPENDENT AND INDEPENDENT SIGNALLING OF EBNA-2 A Thesis Submitted for the Degree of Doctor of Natural Sciences At the Faculty of Biology, Ludwig-Maximilians-Universität München Kristina Grabušić thMünchen, 23 December 2003 Completed at the GSF Research Centre for Environment and Health GmbH Institute for Clinical Molecular Biology and Tumour Genetics, München First Examiner: PD Dr. Bettina Kempkes Additional Examiners: PD Dr. Ruth Brack-Werner Prof. DrHans-Ulrich Koop ofDr. Walter Schartau thDate of the oral examination: 10 May 2004 Mojim roditeljima (To my parents) TABLE OF CONTENTS 1. Introduction 1 1.1. EBNA-2, an important tool of Epstein-Barr Virus 1 1.1.1. Epstein-Barr Virus 1 1.1.2. B-cells are essential for establishment and persistence of EBV infection 1 1.1.3. EBNA-2 takes part in the initial proliferating phase of EBV life cycle in B-cells 2 1.1.4. EBV guides proliferation and differentiation of B-cells by multiple latency programmes 3 1.1.5. In vitro EBV infection of B-cells: a model to study EBV latent genes 4 1.1.6. EBNA-2, the central regulator of transcription in latently infected B-cells 4 1.1.7. EBNA-2 associated disorders 5 1.1.8. EBV associated diseases 6 1.