Random survey for RHD alleles among D positive Europeans [Elektronische Ressource] / Qing Chen

Aus der Medizinischen Klinik und Poliklinik der Universität Ulm Abteilung Transfusionsmedizin (Direktor: Prof. Dr. med. H. Schrezenmeier) Random survey for RHD alleles among D positive Europeans Dissertation for the attainment of the Doctor Degree of Medicine (Dr. med.) at the Faculty of Medicine, University of Ulm Presented by Qing Chen born in Nanjing, Jiangsu, P. R. China 2004 Amtierender Dekan: Prof. Dr. med. Klaus-Michael Debatin 1. Berichterstatter: Priv.-Doz. Dr. med. Willy A. Flegel 2. Berichterstatter: Priv.-Doz. Dr. med. Michael Schmitt Tag der Promotion: 10 December 2004 Content Content 1 Introduction…………………………………………………………………... 1 1.1 Rh blood group system……….…………………………………...…………….. 1 1.2 Clinical importance of the antigen D...……………………………...…………. 3 1.3 Variant of antigen D…….…………………………………...……………………. 3 1.3.1 Weak D …………………………..………………………...……………………... 3 1.3.2 Partial D ……….……………………………………….……………………...….. 4 1.4 Population frequency of variant D in Africans and in Europeans…..……. 4 1.5 Phylogeny of RHD alleles………………………………………………………... 7 1.6 Rationale of the study….…………………………………...……………………. 10 1.6.1 PCR-SSP performed in random donors………………...……………………... 10 1.6.1 Systematic nucleotide sequencing of RHD exon 5 in random donors…….... 12 1.7 The aim of the study…….…………………………………...…………………… 14 2 Materials and Methods……………………………………………………… 15 2.