Quantifying adhesive interactions between cells and extracellular matrix by single-cell force spectroscopy [Elektronische Ressource] / vorgelegt von Anna Verena Taubenberger

TECHNISCHE UNIVERSITÄT DRESDEN Fakultät Maschinenwesen Quantifying adhesive interactions between cells and extracellular matrix by single-cell force spectroscopy DISSERTATION Zur Erlangung des akademischen Grades Doktoringenieur (Dr.-Ing) vorgelegt von Anna Verena Taubenberger geboren am 27.03.1980 in Kulmbach Dresden, 04.05.2009 Gutachter/ thesis reviewers: Prof. Daniel J. Müller Prof. Anthony Hyman Prof. Dietmar W. Hutmacher Tag der Verteidigung/ Day of thesis defense: 07.10.2009 Die Projekte, die Inhalt der Dissertation sind, wurden in der Zeit von 06/2005 bis 04/2009 in der Arbeitsgruppe für zelluläre Maschinen am Biotechnologischen Zentrum der TU Dresden bearbeitet. Betreuender Hochschullehrer war Prof. Daniel J. Müller. The projects that are part of this thesis were conducted between 06/2005 and 04/2009 in the group of cellular machines at the Center of Biotechnology of the TU Dresden. The thesis supervisor was Prof. Daniel J. Müller. Contents PageAbbreviations, symbols and units 1Summary (english and german) 4Chapter 1. Background- Interactions between cells and extracellular matrix 81.1 The extracellular matrix (ECM) 81.1.1 Composition 81.1.2 Effects of ECM on cellular functions 121.1.3 ECM receptors 131.2 Integrins 141.2.1 General aspects 141.2.2 Integrin structure 151.2.3 Integrin regulation 171.2.