Protein folding dynamics [Elektronische Ressource] : single-molecule studies of ribonuclease HI on biocompatible surfaces / vorgelegt von Elza Kuzmenkina

Protein Folding Dynamics Single-Molecule Studies of Ribonuclease HI on Biocompatible Surfaces Dissertation zur Erlangung des Doktorgrades Dr. rer. nat. der Fakultät für Naturwissenschaften der Universität Ulm vorgelegt von Elza Kuzmenkina geborene Amirgoulova aus Narofominsk, Russland Ulm, 2005 Universität Ulm, Abteilung Biophysik Oberer Eselsberg 1 D-89069, Ulm Amtierender Dekan: Prof. Dr. K.-D. Spindler 1. Berichterstatter: Prof. Dr. G. U. Nienhaus 2. Berichterstatter: Prof. Dr. M. Pietralla Tag der Promotion: 10. November 2005 Contents 1 Introduction ................................................................................................................... 4 1.1 Why study protein folding? .................................................................................... 4 1.2 Why study folding on the single-molecule level? .................................................. 6 1.3 FRET as a spectroscopic ruler to study biomolecules............................................ 7 1.4 Why we need to immobilize proteins on biocompatible surfaces.......................... 9 1.4.1 Protein-based surfaces ................................................................................... 11 1.4.2 PEG-based surfaces ....................................................................................... 11 1.