Protein engineering of a Pseudomonas fluorescens esterase [Elektronische Ressource] : alteration of substrate specificity and stereoselectivity / vorgelegt von Anna Schließmann

Protein engineering of a Pseudomonas fluorescens esterase Alteration of substrate specificity and stereoselectivity I n a u g u r a l d i s s e r t a t i o n zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Anna Schließmann geboren am 01.03.1981 in Husum Greifswald, im Mai 2010 II Dekan: Prof. Dr. Klaus Fesser 1. Gutachter: Prof. Dr. Uwe T. Bornscheuer 2. Gutachter: Prof. Dr. Karl-Erich Jaeger Tag der Promotion: 27.07.2010 III „Today is your day! Your mountain is waiting. So… get on your way.” - Theodore Seuss Geisel IV Table of contents Table of contents 1. Introduction.................................................................................................................... 1 1.1. Enantioselectivity ................................................................................................... 1 1.2. Biocatalysis............................................................................................................ 2 1.3. Sources of suitable biocatalysts ............................................................................. 4 1.3.1. Isolation of new enzymes ................................................................