Platelet-derived SDF-1 regulates recruitment, proliferation and differentiation of human CD34_1hn+ progenitor cells [Elektronische Ressource] : implications in patients with acute myocardial infarction / vorgelegt von Konstantinos L. Stellos

Aus der Medizinischen Universitätsklinik und Poliklinik Tübingen Abteilung Innere Medizin III Ärztlicher Direktor: Professor Dr. M. Gawaz Platelet-derived SDF-1 regulates recruitment, proliferation +and differentiation of human CD34 progenitor cells: implications in patients with acute myocardial infarction Inaugural-Dissertation zur Erlangung des Doktorgrades der Medizin der Medizinischen Fakultät der Eberhard-Karls-Universität zu Tübingen vorgelegt von Konstantinos L. Stellos aus Volos, Griechenland 2007 Dekan: Professor Dr. I. B. Autenrieth 1. Berichterstatter: Professor Dr. M. Gawaz 2. Berichterstatter: Professor Dr. U. Quast 3. Berichterstatter: Professor Dr. M. Seyfarth 2 To my parents 3 Contents 1. Introduction………………………………………………………………… 6 1.1 SDF-1 chemokine and its receptor CXCR4……………………… 6 1.2 Progenitor cells in atherosclerosis and angiogenesis…………... 8 1.3 SDF-1 chemokine and progenitor cells…………………………... 8 1.4 Platelet-derived SDF-1 and progenitor cells in atherosclerosis and vascular repair…………………………………………………. 9 1.5 Platelet-derogenitor cells in angiogenesis… 11 1.6 Needs analysis and aim……………….…………………………… 132. Subjects and Methods……..…………………………………………….. 14 2.1 Patients and platelet flow cytometric analysis…………………… 15 2.2 Isolation of platelets………………………………………………… 15 2.