ORFome-based arrays in eukaryotic expression vectors [Elektronische Ressource] : a new approach to screen for the function of viral proteins ; (LANA-1 meets the mediator) / vorgelegt von Maria Roupelieva

ORFome-based arrays in eukaryotic expression vectors – a new approach to screen for the function of viral proteins (LANA-1 meets the mediator) Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Maria Roupelieva Dissertation eingereicht am: 27.01.2005 Erstgutachterin: PD Dr. Bettina Kempkes Zweitgutachter: Prof. Dr. Michael Schleicher Mitberichterstatter: PD Dr. Ruth Brack-Werner Prof. Dr. Martin Parniske Sondervotum: PD Dr. Jürgen Haas Tag der mündlichen Prüfung: 20. 06. 2005 Table of Contents 1. Summary……………………………………………………………………………... 1 2. Introduction……………………………………………………………………….…. 3 2.1. Kaposi’s Sarcoma-Associated Herpesvirus (KSHV)………………..….…... 3 2.1.1. KSHV–associated diseases………………...………………….………... 3 2.1.1.1. Kaposi’s sarcoma (KS) - clinical and morphological features, epidemiology…………………………………………….……………………... 3 2.1.1.2. Primary effusion lymphoma (PEL)……………….…………..…….. 6 2.1.1.3. Multicentric Castleman’s disease (MCD)………………….………. 6 2.1.2. KSHV virion structure……………………………………………………... 7 2.1.3. KSHV genome organization……………………………………………… 8 2.1.4. Gene expression program of KSHV…………………………………….. 10 2.2. Viral modulation of host signalling pathways activating the serum response element (SRE)…………………………………………………..………... 11 2.2.1. Serum Response Element (SRE)………...……………………...……… 11 2.2.1.1.