Optogenetic investigation of nervous system functions using walking behavior and genome wide transcript analysis of synapsin and Sap47 mutants of Drosophila [Elektronische Ressource] / vorgelegt von Nidhi Nuwal

Optogenetic investigation of nervous system functions using walking behavior and genome wide transcript analysis of Synapsin and Sap47 mutants of Drosophila Dissertation zur Erlangung des naturwissenschaftlichen Doktorgrades der Bayerischen Julius-Maximilians-Universität Würzburg vorgelegt von Nidhi Nuwal aus Gangali, India Würzburg, 2010 Eingereicht am: …………………………………… Mitglieder der Promotionskommission Vorsitzender: …………………………………… Erster Gutachter: Prof. Dr. Erich Buchner Zweiter Gutachter: Prof. Dr. André Fiala Tag des Promotionskolloqiums: …………………………………… Doktorurkunde ausgehändigt am: …………………………………… Index Part I 1. Introduction 1.1 Drosophila a geneticist’s tool 1 1.2 Gene expression tools 2 1.2.1 The Gal4 UAS system 2 1.2.2 LexA-LexAop 3 1.3 Optophysiological and optogenetic tools 4 1.3.1 Tools to visualize and monitor neuronal activity 4 1.3.2 Tools to manipulate neuronal activity 6 1.3.2.1 Caged biogenic compounds 7 1.3.2.2 Natural photosensitive proteins 9 1.3.2.3 Small molecule photoswitches 15 1.4 Miscellaneous neuronal manipulation tools 16 1.4.1 Temperature induced neuronal manipulation 16 1.4.2 Sodium activated bacterial channel 17 1.5 Experience dependent modification of behavior 17 1.5.1 Classical conditioning 18 1.5.