On the putative role of Pelota in stem cell differentiation [Elektronische Ressource] / vorgelegt von Aleksandra Kata

On the putative role of Pelota in stem cell differentiation Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultäten der Georg-August-Universität zu Göttingen vorgelegt von Aleksandra Kata aus Łódź, Polen Göttingen 2009 D 7 Referent: Prof. Dr. med Dr. h.c. W. Engel Korreferentin: Prof. Dr. S. Hoyer-Fender Tag der mündlichen Prüfung: TABLE OF CONTENTS TABLE OF CONTENTS TABLE OF CONTENTS i ABBREVIATIONS v 1. INTRODUCTION 1 1.1. The pelota gene 1 1.2. The function of pelota gene in Saccharomyces cerevisiae and Drosophila melanogaster 4 1.3. The phenotype of Pelota knockout mice 5 1.4. Aims of the study 7 2. MATERIALS AND METHODS 8 2.1. MATERIALS 8 2.1.1. Chemicals 8 2.1.2 Solutions, buffers and media 11 2.1.3 Laboratory materials 13 2.1.4 Sterilisation of solutions and equipments 13 2.1.5 Media, antibiotics and agar-plates 13 2.1.5.1 Media for bacteria 13 2.1.5.2 Media for cell and embryo culture 14 2.1.5.3 Antibiotics 15 2.1.5.4 IPTG/X-Gal plate 15 2.1.6 Bacterial strains 15 2.1.7 Plasmids 16 2.1.8 Synthetic oligonucleotides 16 2.1.9 Mouse strains 17 2.1.10 Antibodies 17 2.1.11 Enzymes 18 2.1.