Oligomerisation, localisation and interaction of the sensor histidine kinases DcuS and CitA in Escherichia coli [Elektronische Ressource] / vorgelegt von Patrick Daniel Scheu

Oligomerisation, Localisation and Interaction of the sensor histidine kinases DcuS and CitA in Escherichia coli Dissertation zur Erlangung des Grades „Doktor der Naturwissenschaften“ Am Fachbereich Biologie der Johannes Gutenberg-Universität in Mainz vorgelegt von Patrick Daniel Scheu geb. am 17.06.1980 in Medellín Mainz, November 2009 Dekan: 1. Berichterstatter: 2. Berichterstatter: Tag der mündlichen Prüfung: 11.12.2009 Contents Contents 1. Abstract...............................................................................................................................1 2. Introduction ........................................................................................................................2 3. Materials and methods ....................................................................................................10 3.1 Bacterial strains and plasmids......................................................................................10 3.2 Growth and media ........................................................................................................12 3.3 Molecular genetic methods...........................................................................................18 3.4 Protein-biochemical methods .......................................................................................27 3.5 Physicochemical methods........