Nutrient regulated expression systems of Bacillus licheniformis [Elektronische Ressource] / vorgelegt von Thanh-Trung Nguyen

Nutrient regulated expression systems of Bacillus licheniformis INAUGURALDISSERTATION zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald 2010 vorgelegt von Nguyen Thanh Trung geboren am 26. November 1981 in Hai Duong - Vietnam Greifswald, November 2010 Dekan: Prof. Dr. Klaus Fesser 1. Gutachter 1: Prof. Dr. Thomas Schweder 2. Gutachter 2: Prof. Dr. Peter Neubauer Tag der Promotion: 15. November 2010 SUMMARY SUMMARY The acoABCL and acuABC operons of B. licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the acoABCL operon is controlled by a sigmaL-dependent promoter and the acuABC operon by a sigmaA-dependent promoter. By means of reporter-gene-fusions the temporal control of both promoters was analyzed. Transcription at the acoA promoter is repressed by glucose but induced by acetoin as soon as the preferred carbon source glucose is exhausted. The acuA promoter shows a similar induction pattern but its activity is independent from the presence of acetoin. It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis.