New procedures for the diagnosis of human brucellosis in Mongolia [Elektronische Ressource] / vorgelegt von Zandraa Jamba

New Procedures for the Diagnosis of Human Brucellosis in Mongolia Inauguraldissertation zur Erlangung des Grades eines Doktors der Humanbiologie des Fachbereichs Medizin der Justus-Liebig-Universität Giessen vorgelegt von Zandraa Jamba aus Ulaanbaatar, Mongolei Giessen 2008 I Aus dem Biochemischen Institut des Fachbereichs Medizin der Justus-Liebig-Universität Giessen Geschäftsführender Direktor: Prof. Dr. Klaus T. Preissner Gutachter: Prof. Dr. Ewald Beck Gutachter: Prof. Dr. Rolf Bauerfeind Tag der Disputation: 24.06.2008 II SUMMARY The feasibility of developing immunological and molecular diagnostic tools for routine diagnosis of brucellosis under conditions of a less economically and scientifically advanced country in general and Mongolia in particular was analysed. Brucellosis is a major healthcare issue in Mongolia for both, humans as well as lifestock farming, leading to enormous economic losses every year. Diagnosis of the disease is demanding and insufficiently specific with the available tests. Using genomic sequences of the most important Brucella strains, new diagnostic procedures have been developed by means of molecular biotechnology. They include the production of several recombinant proteins as antigens in immunological assays such as ELISA.