Nanoscale imaging of restricted cell membrane receptor diffusion [Elektronische Ressource] / von Christian Tischer

Nanoscale imaging of restricted cell membrane receptordi usionDISSERTATIONzur Erlangung des akademischen Gradesdoctor rerum naturalium(Dr. rer. nat.)im Fach Biophysikeingereicht an derMathematisch-Naturwissenschaftlichen Fakultat IHumboldt-Universitat zu BerlinvonHerr Dipl.-Phys. ChristianTischergeboren am 13.12.1973 in StuttgartPrasident der Humboldt-Universitat zu Berlin: Prof. Dr. Jurgen MlynekDekan der Mathematisch-Naturwissenschaftlichen Fakultat I:Prof. Thomas Buckhout, PhDGutachter:1. Prof. Dr. Reinhart Heinrich2. Prof. Dr. Philippe Bastiaens3. Prof. Dr. Ernst-Ludwig FlorinTag der mundlichen Prufung: 9. Juni 2005iiAbstractIntheworkpresented,anovelimagingtechnique(TNIM-ThermalNoiseImagingMicroscopy)was developed for the purpose of studying nanoscale di usive motion in heterogeneous me-dia. TNI-Microscopy was specically used to investigate if the mobility of cell membranereceptors is inuenced by lateral membrane nanostructures. The direct investigation of thesetwo-dimensional ultrastructures in living cells was up to now hampered because of lackingmicroscopy techniques. In TNI-Microscopy, the di usive motion of a nanoparticle is limitedto a small volume, making it possible to observe constraints on the particle’s motion. Thus,the particle can be used to sample nanostructures. Concomitant, it is also recorded how thedynamics of the particle’s motion are inuenced by hydrodynamic coupling to the sampledstructures.