Molecular studies on plants to enhance their stress tolerance [Elektronische Ressource] / by Alaa El-din A. Helaly

From the University of Potsdam and the Max-Planck Institute of Molecular Plant Physiology Molecular studies on plants to enhance their stress tolerance DISSERTATION A thesis submitted to the Institute of Biochemistry and Biology Faculty of Mathematics and Natural Sciences University of Potsdam For the degree of Doctor of natural sciences (doctor rerum naturalium) in molecular biology By Alaa El-din A. Helaly Potsdam 2004 Contents 1 Introductiuon 1 1.1 Transcription factors in eukaryotes 1 1.2 Transcription factor domain 2 1.3 Zinc finger proteins 3 1.4 AtSTO1 as a putative transcription factor 6 1.5 Regulatory role of transcription factor proteins under stress 7 1.6 Mechanism of salt stress and plant response 101.7 Proline biosynthesis in plants under stress 121.8Anthocyanin biosynthesis 14 2 Aim of this work 16 3Materials and methods 17 3.1 Chemicals and enzymes 173.2 Oligonucleotides 173.3 Vectors and plasmids 183.4 Bacteria 183.4.1 Escherichia coli 183.4.2 Agrobacterium tumefaciens 183.5 Plants 183.6Growth conditions 183.6.1 Bacteria 183.6.2 Tobacco BY2 cells 193.6.3 Plants 193.6.3.1 Transformation in A. thaliana 193.6.3.2 Seed sterilization193.6.3.2 Tissue culture 193.6.3.3 Phytotron 203.7 Molecular methods203.7.1 Cloning and sequence 203.7.2 Amplification of DNA fragments via polymerase chain reaction 21 i3.7.