Molecular phylogeography of the European coastal plants Crithmum maritimum L., Halimione portulacoides (L.) Aellen, Salsola kali L. and Calystegia soldanella (L.) R. Br. [Elektronische Ressource] / Rami M. Arafeh

Molecular phylogeography of the European coastal plants Crithmum maritimum L., Halimione portulacoides (L.) Aellen, Salsola kali L. and Calystegia soldanella (L.) R. Br. Dissertation zur Erlangung des Grades “Doktor der Naturwissenschaften” Am Fachbereich Biologie der Johannes Gutenberg-Universität in Mainz Rami M. Arafeh geb. in Hebron, Palästina D 77 Tag der mündlichen Prüfung: 11. Februar 2005 D 77 CONTENTS 1. Introduction 1 2. Materials and Methods 5 2.1 Study Species 2.1.1 Crithmum maritimum 2.1.2 Halimione portulacoides6 2.1.3 Salsola kali 7 2.1.4 Calystegia soldanella 10 2.2 Sampling of plant material 13 2.3 Extraction and purification of DNA 2.4 Amplified fragment length polymorphism (AFLP) fingerprinting 2.4.1 AFLP gel processing, evaluation and generating the binary data matrix 15 2.4.2 AFLP data analysis 15 2.5 Amplification of the internal transcribed spacer (ITS) 16 2.5.1 Purification of PCR product 19 2.5.2 Sequencing reaction 19 2.5.3 Cloning of PCR product 19 2.5.4 Sequences editing and alignment 20 2.5.5 Maximum Parsimony (MP) analysis 20 2.5.6 Median Joining (MJ) network 21 3. Results 22 3.1 Crithmum maritimum 3.1.1. AFLP analysis 3.1.